![]() Significant drop in AFI was noted at two-week intervals. There was a gradual decline of AFI values as the gestational age approached term. The 5th percentile cut-off was 8.7 cm at 40 weeks. Starting from 34 weeks till 40 weeks, 50 ultrasound measurements were available at each gestational age. Cohen's d coefficient was used to examine the magnitude of change at different time intervals. Percentile curves (5th, 50th, and 95th centiles) were constructed for comparison with other studies. Statistical analysis was done using SPSS software (Version 16, Chicago, IL). Amniotic fluid index was obtained by adding these four measurements. Linear array transabdominal probe was used to measure the largest vertical pocket (in cm) in perpendicular plane to the abdominal skin in each quadrant. For the purpose of AFI measurement, the uterine cavity was arbitrarily divided into four quadrants by a vertical and horizontal line running through umbilicus. Women with gestational or overt diabetes mellitus, hypertensive disorders of the pregnancy, prelabour rupture of membranes, and congenital anomalies in the foetus and those who delivered before 40 completed weeks were excluded from the study. Only low risk singleton pregnancies with accurately established gestational age who were available for all weekly scan from 34 to 40 weeks were included in the study. The trend of amniotic fluid volume was studied with advancing gestational age. A prospective estimation of AFI was done in 50 healthy pregnant women from 34 to 40 weeks at weekly intervals. To establish reference standards for AFI for local population after 34 weeks of pregnancy and to decide an optimal scan interval for AFI estimation in third trimester in low risk antenatal women. However, before deciding the cut-off standards for abnormal values for a local population, what constitutes a normal range for specific gestational age and the ideal interval of testing should be defined. ![]() Very low values are associated with intrauterine growth restriction and renal anomalies of fetus, whereas high values may indicate fetal GI anomalies, maternal diabetes mellitus, and so forth. Amniotic fluid index (AFI) is one of the major and deciding components of fetal biophysical profile and by itself it can predict pregnancy outcome. MMK and IC are the guarantors of this work and, as such, had full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.Background. MMK and IC wrote the manuscript, and all authors contributed toward data interpretation and critically revising the paper and accepted the final version. LE performed the studies on mitochondrial isolates and prepared samples for lipidomic analyses. MMK, AF and JS carried out STB and endothelium TEM analyses. MMK performed half of IF experiments and participated in WBs together with AT and AF and analyzed clinical data. JA carried out the cytotrophoblast transmission electron microscopy (TEM) analyses, western blot (WB) and half of immunofluorescence (IF) experiments. Download Spss 17 Full Version For Windows 7 32 Bit
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